Enhance your current IHC with high-plex profiling data
- Multiplex up to 800 RNA or protein targets on one FFPE slide in a single pass
- Completely non-destructive: sample is only touched by light
- Up to 6 logs (base 10) dynamic range with limit of detection down to single-cell resolution
- Significantly fewer steps and less hands-on time than TSA-based multiplexing
- Validation of novel high-plex protein spatial profiling quantitation based on NanoString's Digital Spatial Profiling (DSP) technology with quantitative fluorescence (QIF)
- Spatially resolved, multiplexed digital characterization of protein and mRNA distribution and abundance in formalin-fixed, paraffin-embedded (FFPE) tissue sections based on NanoString’s Digital Spatial Profiling (DSP) technology: applications to immuno-oncology (IO) and tumor heterogeneity
- Spatially resolved, multiplexed digital characterization of protein and mRNA abundance in FFPE tissue sections: application to immuno-oncology
- Spatially resolved, multiplexed digital characterization of protein and RNA expression in FFPE tissue sections
- Spatially resolved, multiplexed digital characterization of protein and RNA distribution and abundance in FFPE tissue sections
- A new approach for immuno-oncology biomarker discovery: high-plex, spatial protein profiling based on NanoString digital quantification
- Multidimensional spatial characterization of the tumor microenvironment in synchronous melanoma metastases yields insights into mixed responses to therapy
- (Neo-)adjuvant ipilimumab + nivolumab (IPI+NIVO) in palpable stage III melanoma: Updated data from the OpACIN trial and first immunological analyses
In contrast to the sequential analysis of multi-target immunohistochemistry (IHC) slides, NanoString's DSP technology samples all analytes on a single FFPE slide. This not only shortens processing time and simplifies data analysis, but also provides a higher multiplexing capacity of up to 800 targets and a wider detection range - all with spatial context.
Based on NanoString's proprietary barcoding technology, the DSP platform measures local protein levels, and can be combined with RNA expression giving researchers the ability to spatially resolve RNA when suitable antibodies do not exist. The platform includes imaging and fluidic components to capture spatial context, and current nCounter® instruments provide the quantification.
Protein detection is enabled with primary antibodies that are covalently attached via a UV photocleavable linker to DNA indexing oligos. Following antigen retrieval, FFPE tissue samples are stained with a multiplexed cocktail of labeled antibodies, and DNA oligos are subsequently released by UV light exposure across Regions of Interest (ROIs). The liberated DNA oligos are then hybridized to optical barcodes for quantitation on an nCounter® system.
How it works
Process: Apply high-plex antibody cocktail
View: Use visible wavelength low-plex imaging to establish tumor "geography." Select regions-of-interest (ROIs) for high-plex profiling
Profile: UV-release high-plex oligo tags at selected ROIs
Plate: Store released tags in microtiter plate, index, and hybridize to barcodes
Digitally count up to 1 million data points per ROI
Analyze the data with nSolver™ Advanced Analysis Software
For Research Use Only. Not for use in diagnostic procedures.